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KMID : 0811720140180010073
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Korean Journal of Physiology & Pharmacology
2014 Volume.18 No. 1 p.73 ~ p.78
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Bcl-2 Knockdown Accelerates T Cell Receptor-Triggered Activation-Induced Cell Death in Jurkat T Cells
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Lee Yun-Jung
Won Tae-Joon
Hyung Kyeong-Eun
Lee Mi-Ji
Lee Ik-Hee
Go Byung-Sung
Hwang Kwang-Woo
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Abstract
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Cell death and survival are tightly controlled through the highly coordinated activation/inhibition of diverse signal transduction pathways to insure normal development and physiology. Imbalance between cell death and survival often leads to autoimmune diseases and cancer. Death receptors sense extracellular signals to induce caspase-mediated apoptosis. Acting upstream of CED-3 family proteases, such as caspase-3, Bcl-2 prevents apoptosis. Using short hairpin RNAs (shRNAs), we suppressed Bcl-2 expression in Jurkat T cells, and this increased TCR-triggered AICD and enhanced TNFR gene expre-ssion. Also, knockdown of Bcl-2 in Jurkat T cells suppressed the gene expression of FLIP, TNF receptor-associated factors 3 (TRAF3) and TRAF4. Furthermore, suppressed Bcl-2 expression increased
caspase-3 and diminished nuclear factor kappa B (NF-κB) translocation.
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KEYWORD
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AICD, Apoptosis, Bcl-2, shRNA, T cell
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